TABLE 2.
Primers for cloning human Nrf2, Keap1, and Siah2 and for construction of Nrf2 mutants
| Factor | Primer no. | Sequence |
|---|---|---|
| Nrf2 | 1 | AAG GAT CCA TCA TGA TGG ACT TGG AGC T |
| 2 | AAG GTA CCA TTC AAC ATA CTG ACA CTC C | |
| 3 | ATA GTC GAC CAT GAT GGA CTT GGA GC | |
| 4 | AGA CTC GAG AAC ATA CTG ACA CTC CAA TG | |
| Keap1 | 5 | ATG GAT CCC CCA TGC AGC CAG ATC CCA G |
| 6 | TTA AGC TTA ATC AAC AGG TAC AGT TCT GCT | |
| 7 | ATTA AGC TTC CCA TGC AGC CAG ATC C | |
| 8 | CTG GAT CCT CAA CAG GTA CAG TTC TG | |
| Siah2 | 9 | ATG GAT CCG CGA TGA GCC GC |
| 10 | GCG GAA TTC GAA AGT CAC ATC ATG GAC | |
| pCMV | 11 | CAG GAT CCA TGG CAT CAA TGC AGA AG |
| Nrf2(93–604) | 12 | ATA GTC GAC CCA GTC AGA AAC CAG TG |
| V172A | 13 | TCT GTT GCT CAG GCA GCC CCT GTT GAT TTA |
| 14 | TAA ATC AAC AGG GGC TGC CTG AGC AAC AGA | |
| V172A/P174A | 15 | GTT GCT CAG GCA GCC GCT GTT GAT TTA GAC |
| 16 | GTC TAA ATC AAC AGC GGC TGC CTG AGC AAC | |
| S40N | 17 | GAG AAG TAT TTG ACT TCA ATC AGC GAC GGA |
| 18 | TCC GTC GCT GAT TGA AGT CAA ATA CTT CTC | |
| S40D | 19 | GAG AAG TAT TTG ACT TCG ATC AGC GAC GGA |
| 20 | TCC GTC GCT GAT CGA AGT CAA ATA CTT CTC |