FIGURE 2.

Pharmacological inhibition of ERK activation. M1R cells stably expressing tetracycline-inducible FLAG-TRPC6 R895C were treated without (−Tet) or with tetracycline (all other samples) overnight before being treated with the indicated inhibitors for 3 h prior to cell lysis. A, Western blot analysis of phosphorylated ERK (P-Erk), total ERK1/2, and FLAG-TRPC6. B, average normalized phospho-ERK levels in cells exposed to various inhibitors. One-way ANOVA with Dunnett's multiple comparison test was used; *, p < 0.05 versus DMSO-treated; n = 3. Table 1 summarizes the major targets of inhibitors used. Error bars, S.E.