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. 2013 May 3;288(25):18407–18420. doi: 10.1074/jbc.M113.463059

FIGURE 3.

FIGURE 3.

Effect of KN-92 and KN-93 on ERK activation by TRPC6 R895C. M1R cells expressing FLAG-TRCP6 R895C, or uninduced cells (−Tet) were treated with the indicated inhibitors or DMSO as a carrier control for 3 h prior to cell lysis. A, Western blot analysis of phospho-ERK (P-Erk), ERK, and FLAG-TRPC6 levels. B, average normalized phospho-ERK levels in cells exposed to the indicated inhibitors. One-way ANOVA with Tukey's multiple comparison test was used; **, p < 0.001 versus all other conditions; all other comparisons, p > 0.05; n = 4. C, Western blot analysis of 293T cells transfected with HA-TRPC6 R895C and either GFP (−), GFP-AC3 inhibitor (I), or control (C) constructs, as indicated. Error bars, S.E.