FIGURE 5.

Expression of miR-766 was higher in older subjects. A, relative endogenous expression of miR-766 was determined in HDFs from young versus old age groups by real-time PCR with 6 HDFs both the groups. B, relative endogenous expression of SEPT6 was determined in HDFs from two age groups by real-time PCR. C, schematic of SEPT6 promoter subdivided to identify promoter acetylation and binding of transcription factor(s) to subregions as described under “Experimental Procedures.” D, ChIP was performed at the SEPT6/miR-766 promoter using H3K9Ac and H3 antibodies. H3K9 acetylation at the SEPT6/miR-766 promoter is shown relative to untreated control samples and normalized to total H3 levels. E, SP1 occupancy at the SEPT6/miR-766 promoter was measured for HDFs of older subjects versus younger subjects. Results (A, B, and C) are presented as mean ± S.E. At least four independent experiments were performed. *, p < 0.05.