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. 2013 May 6;288(25):18484–18493. doi: 10.1074/jbc.M113.458158

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Reprogramming of microRNA expression by wild-type and HBx-deficient HBV. A, viral replication was monitored in HepG2 cells transfected with wild-type or HBx-deficient HBV. Time course HBV replication was determined on the basis of the production of the s and e antigens. HBx-deficient HBV showed only a minor defect after 72 h. B, a panel of microRNAs implicated previously in the regulation of HBV replication were analyzed in HepG2 cells infected with wild-type and HBx-deficient HBV. HBx-deficient HBV only showed a reduced induction of miR-29a and reduced suppression of miR-Let-7a compared with wild-type HBV. *, p < 0.05; **, p < 0.01 on the basis of experiments performed in triplicate.