FIGURE 6.

RNAi-mediated depletion of PGI. A and B, growth of the wild-type (WT), Δmec, ^RNAiPGI, and Δmec/^RNAiPGI cell lines in glucose-rich (A) and glucose-depleted (B) medium. The control of RNAi efficiency by Western blot analysis is shown above the growth curves (PFR A/C, paraflagellar rod proteins A and C). PGI protein levels after 4 days of induction were quantified as 15 and 13% (A) or 10 and 11% (B) for the single and double mutants, respectively. C, induction of oxidative stress by different concentrations of XOX in glucose-depleted medium. RNAi was induced with tetracycline 72 h prior to addition of XOX. Error bars represent the S.E. of three independent experiments.