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NBD2* degradation utilizes Cdc48p. Cycloheximide chases were performed as described under “Experimental Procedures” in BY4742 and cdc48-2 yeast following a 2-h temperature shift to 37 °C. Data represent the means ± S.E. (error bars) for four independent experiments, each performed in triplicate; *, p ≤ 0.036.
