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. 2013 May 7;288(25):18506–18520. doi: 10.1074/jbc.M113.475905

FIGURE 7.

FIGURE 7.

Hsp70 function contributes to NBD2* polyubiquitination. In vitro ubiquitination assays were performed using cytosol prepared from SAN1UBR1 or san1Δubr1Δ yeast (A) or cytosol prepared from SSA1 or ssa1-45 yeast (B) following a 45-min shift to 37 °C. Graphs represent the means ± S.E. (error bars) from five independent experiments performed with replicates utilizing cytosol made three separate times (*, p ≤ 0.006 (SSA1) and p ≤ 0.0001 (SAN1UBR1)). Molecular weight markers (× 10−3) are shown to the left.