TABLE 3.

Survey of the degradation requirements for select CytoQC substrates

The table demonstrates the factors that have been tested for their involvement in the CytoQC of the indicated substrates, and a brief description of the substrates is also provided. The assays used to examine the contribution of each factor represent a mixture of both pulse-chase and cycloheximide chase analyses. +, a requirement; (+), a slight requirement; −, no requirement; ND, not determined.

Substrate	Description	Hsp70 (Ssa1p)	Hsp110 (Sse1p)	Hsp40 (Ydj1p)	E3 (Doa10p)	E3 (San1p)	E3 (Ubr1p)	Cdc48p Complex	Reference/Source
HA-NBD2*	C-terminal nucleotide binding domain of Ste6p*	+	+	+	−	+	(+)	+	This Study
ΔssCPY*	CPY* (ERAD substrate) lacking ER signal sequence	+	−	+	−	ND	ND	−	Ref. 68
ΔssCPY*		+	+	+	ND	+	(+)	ND	Ref. 27
Ura3p-CL1	CL1 degron fused to Ura3p	+	ND	+	+	ND	ND	(+)	Ref. 2
Matα2	Short-lived transcription factor	ND	ND	ND	+	ND	ND	−	Ref. 24 and 25
ΔssPrA	Vacuolar proteinase A lacking signal sequence	+	+	+	ND	+	(+)	ND	Ref. 26
Δ2GFP	GFP with a disrupted β-barrel	+	+	+	ND	+	(+)	ND	Ref. 26
tGnd1	Truncated phosphogluconate dehydrogenase	+	+	ND	ND	(+)	+	ND	Ref. 27
VHL	Tumor suppressor protein with point mutation	+	+	−	ND	ND	ND	ND	Ref. 21