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. 2013 May 6;288(25):18546–18560. doi: 10.1074/jbc.M112.446591

FIGURE 5.

FIGURE 5.

Decreased Oct4, but not Nanog expression, is rescued by silencing Cdx2 expression. A, CDX2 mRNA expression in control hESCs and hESCs with CDX2 shRNAs. B, decreasing CDX2 expression in hESCs using CDX2 shRNA for 4 days restores expression of OCT4, but not NANOG, as assessed by immunoblots. C, immunofluorescence of CDX2 or NANOG expression in control hESCs and hESCs with decreased SMAD2 expression at day 8 after shRNA infection. Noggin or BMP4 was added from day 3 to day 8. DAPI served as a nuclear counter-stain. D, NANOG and CDX2 mRNA expression in control hESCs and hESCs with decreased SMAD2 expression, in the presence or absence of Noggin or BMP4 between day 3 and day 8 after infection. n = 3 assays. *, p < 0.05; **, p < 0.01, compared with control cells by Student's t tests; ***, p <0.005.