FIGURE 4.

Inhibition of PvSUB1r, PfSUB1, and PbSUB1 enzymatic activity by Cpd2. Dose-dependent inhibition of PvSUB1r (A), PfSUB1r (B), and PbSUB1 (D) by Cpd2. Results presented are the mean (± S.D.) of at least three independent experiments. Lineweaver-Burk representation of PvSUB1r inhibition by Cpd2 (C) suggests inhibition of PvSUB1 via a substrate competitive mechanism. The enzymatic assay was performed on four different concentrations of Cpd2 (50 to 0.4 μm, as indicated) and on five different concentrations of the PvSUB1-FR substrate ranging from 40 to 5 μm.