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. 2013 May 6;288(25):18612–18623. doi: 10.1074/jbc.M112.440859

FIGURE 1.

FIGURE 1.

SIKE is a mixed-type inhibitor of TBK1. A, Michaelis-Menten plot of TBK1-mediated phosphorylation of IRF3 with saturating ATP (100 μm), pre-activated TBK1 (4.93 nm), and IRF3 varied from 0.042 to 20.8 μm. Data were fit to a 2-parameter rectangular hyperbola (SigmaPlot). B, Michaelis-Menten plots of TBK1-mediated phosphorylation of IRF3 with increasing SIKE (0–83 nm) fit to a single rectangular 2-parameter hyperbola curve (SigmaPlot). C, Michaelis-Menten plot of TBK1-mediated phosphorylation of IRF3 with saturating IRF3 (20.8 μm), pre-activated TBK1 (4.93 nm), and ATP varied from 0 to 150 μm. Data were fit to a 2-parameter rectangular hyperbola (SigmaPlot). D, Michaelis-Menten plots of TBK1-mediated phosphorylation of IRF3 (20.8 μm) with ATP-varied (0.08–150 μm) and increasing SIKE (0–83 nm) fit to a single rectangular, 2-parameter hyperbola curve (SigmaPlot). Circle, no SIKE; triangle, 20.8 nm SIKE; square, 83 nm SIKE.