TABLE 2.

Parameters for SIKE inhibition of TBK1-mediated IRF3 phosphorylation

SIKE, nmb	Ki, appa
	Intercept		Slope
	20.8 nm	83.3 nm	20.8 nm	83.3 nm
ATP	71.7 ± 5.7	41.2 ± 5.0	589.7 ± 4.6	243.4 ± 5.3
IRF3 (1.7–20.8 μm)c	58.1 ± 8.1	100.4 ± 4.7	ND	88.9 ± 3.5
IRF3 (0.042–0.42 μm)	93.4	133.6	79.7	109.4

^a K_{i, app} refers to parameter describing K_i for all IRF3 phosphorylation sites assayed. K_i derived from intercept_(Inh) = intercept_(none)(1 + [Inh]/K_i), where Inh = SIKE concentrations and intercept derived from linear regression of LWB plots.

^b SIKE construct included residues 72–207.

^c IRF3 construct included residues 173–427 and parameters derived from data at listed concentrations.