TABLE 3.

Parameters for mutant SIKE inhibition of TBK1-mediated IRF3 phosphorylation

SIKE constructa	Ki, appb
	nm
WT	350 ± 16
72–184	196 ± 7.9
113–207	363 ± 52
S6E	900 ± 56
S6A	77 ± 6
S4A	140 ± 7
S2A	368 ± 14
S185A	41 ± 8

^a SIKE construct included residues 72–207 except where explicitly stated.

^b K_{i, app} derived from fit of 2-parameter rectangular hyperbola to % inhibited V_o-pIRF3 μm/min versus SIKE concentration.