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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2013 May 25;21(9):1843–1849. doi: 10.1002/oby.20329

Figure 1.

Figure 1

FFAs increase cytokine expression in human preadipocytes. Human sc (HSP) and omental (HOP) preadipocytes were serum-starved for 12 hours then pretreated with or without 10 nM IGF-I for 1 hr followed by 0.1mM FFAs for 4 hours prior to RNA isolation. RNA was analyzed by quantitative RT-PCR using Syber green and primers for TNFα, MCP-1 and IL-6. Data are presented as mean +SE, n= 3, p<0.05 in FFA-treated compared to IGF-I plus FFA-treated.