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FFA and MALP-2, but not Pam3CSK4 increase phosphorylation of JNK. Human sc preadipocytes were serum-starved overnight and treated with Pam3CSK4 1µg/ml or 10µg/ml, MALP-2 2µg/ml or 5µg/ml or FFAs 0.1mM for 20 minutes followed by WB analysis of cell lysates using phospho and total JNK, ERK and p38 antibodies. Representative blot is shown; this was repeated twice with similar results.
