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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2013 May 25;21(9):1843–1849. doi: 10.1002/oby.20329

Figure 4.

Figure 4

FFA-induced cytokine expression is decreased by p38 and JNK inhibition. Human sc preadipocytes were serum-starved for 12 hours then pretreated for 1 hour with control, 50µM ERK inhibitor PD098059, 50µM p38 inhibitor SB20358 or 50µM JNK inhibitor SP600125 prior to 0.1mM FFAs for 4 hours. RNA was isolated for analysis by quantitative RT-PCR using Syber green and primers for A. TNFα expression: data are presented as mean +SE, n=3, p<0.001 FFA-treated compared to SB20358 plus FFAs; p<0.0001 FFA-treated compared to SP600125 plus FFAs and B. MCP-1 expression: data are presented as mean +SE, n=3, p<0.001 FFA-treated compared to SB20358 plus FFAs; p<0.001 FFA-treated compared to SP600125 plus FFAs.