Fig. 6.

Effect of Cyp11a1 on primary pituitary tumor cell proliferation. a Infection of rat primary pituitary tumor cells with lentiviral vectors expressing shCyp11a1-GFP or GFP only (MOCK). b Primary pituitary tumor cells from three mutant rats were infected with the lentiviral vectors as in “a” and the level of Cyp11a1 was analyzed by qRT-PCR as indicated in the legend of Fig. 1, and is reported relative to the expression level in mock-transfected cells arbitrarily set to 100. c In samples parallel to “b”, cell proliferation was assessed 72 h after infection using the WST-1 assay. Data were analyzed independently with six replicates each and were expressed as the mean ± SEM. d In samples parallel to “c”, the expression of p21 was assessed by qRT-PCR as indicated in the legend of Fig. 1, and is reported relative to the expression level in mock-transfected cells arbitrarily set to 1. e Caspase 3/7 activity was measured in samples in parallel to “c” to monitor apoptosis in GH3 cells 72 h post-infection. *P < 0.05, **P < 0.01 versus MOCK