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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):49–60. doi: 10.1042/BJ20121598

Figure 1. Lipin1 enhances PPARγ transcriptional activity.

Figure 1

(A) NIH 3T3 cells were cotransfected with 3× PPRE-tk luciferase constructs, PPARγ and RXR with a combination of pSG5-KH2M1 (Mock), pSG5-lipin1α or lipin1β. (B) PC3 cells were transfected with 3× PPRE-tk luciferase constructs along with empty vector, pSG5-lipin1α or lipin1β. Rosiglitazone (Rosi; 1 μM) was added 1 day after transfection. Relative luciferase activity was calculated as described in Experimental section.