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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):49–60. doi: 10.1042/BJ20121598

Figure 5. The mechanism of lipin1-mediated PPARγ activation differs from PPARα activation.

Figure 5

(A) Expression vectors for GAL4-DBD–PPARα (left-hand panel) or the GAL4-DBD–PPARγ (right-hand panel) fusion proteins and pFR-Luc were transfected into NIH 3T3 cells along with pcDNA-PGC-1α and/or pSG5-lipin1, as indicated. (B–D) PPRE-tk-luciferase and expression vectors for PPARα (left-hand panels), PPARγ (right-hand panels), RXRα, PGC1α, lipin1, lipin1 mutants (ΔTAD, LL728FF and LL888FF) or lipin2 were transfected into NIH 3T3 cells as indicated. Rosi., rosiglitazone.