Fig. 1.
(A) Schematic representation of the sequences of HA-tagged full-length human A1 (22 kDa), A1ΔC (19.8 kDa) – A1 mutant with a deletion of the C-terminus domain, A1 BH4 (20.1 kDa) – A1 mutant with a deletion of the BH4 domain, A1 (17.8 kDa) – A1 mutant with a deletion of the C-terminus and the BH4 domains that were cloned in pAC plasmids. (B) Expression of A1 and A1 mutants in bovine aortic endothelial cells (BAEC) following their transfection with the respective plasmids, as demonstrated by Western blot analysis using anti-HA antibody. Molecular weight markers are labeled on the left side of the figures.