Fig. 3.

Overexpression of A1ΔC but not full length A1 prevents TNF-induced IκBα degradation in HUVEC and HDMEC. Cell lysates from non-transduced (NT), rAd.A1, rAd.A1ΔC or rAd.βgal-transduced cells (A) HUVEC, and (B) HDMEC treated with TNF (200 U/ml) for 15 and 120 min were immunoblotted with anti-IκBα antibody. Results demonstrate that overexpression of A1 does not affect TNF-induced degradation and loss of IκBα in all EC tested. However, overexpression of A1ΔC inhibits TNF-induced IκBαdegradation in both HUVEC and HDMEC. Immunoblotting with anti-HA and anti-βactin antibodies was used to confirm transgene expression and correct for loading, respectively. Data shown are representative of 3 independent experiments for each endothelial cell type.