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. Author manuscript; available in PMC: 2013 Jun 23.
Published in final edited form as: J Immunol. 2010 Sep 27;185(9):5503–5511. doi: 10.4049/jimmunol.1001258

FIGURE 3.

FIGURE 3

Transcellular biosynthesis of LTB4 in FLS/neutrophil co-cultures. A, LTB4 formation after stimulation of WT FLSs (8 × 104 cells per milliliter) and WT neutrophils (8 × 103 cells per milliliter) either alone or in coculture after stimulation with 1 μM A23187 for 30 min. B, LTB4 formation after coculture of 5-LO–/– neutrophils and WT FLSs in the presence of 1 μM A23187 for 30 min. C, LTB4 formation after coculture of LTA4H–/– neutrophils and WT FLSs in the presence of 1 μM A23187 for 30 min. LTB4 was analyzed as described in Materials and Methods. Values represent the mean ± SEM of data pooled from three independent experiments. The p values are indicated on the top of the each bar. The assay limit of detection is denoted by the horizontal dotted line.