FIGURE 1.

Clinical grade of inflammation and upregulation of RAGE ligands in DTH. (A) DTH time course in WT (n = 20) and RAGE^−/− (n = 19) mice; data from three independent experiments. The grading of the clinical score was performed as described in Materials and Methods. (B) Protein extract from footpads of WT mice (one control, one DTH) was passed along Ni-TED-Resin with attached soluble RAGE, and eluted proteins were immunoblotted with anti-S100B, anti-CD6, and anti-HMGB-1 as described in Materials and Methods. (C) Western blot analysis of total protein extract of WT and RAGE^−/− footpads (Control = Ctrl and DTH) using anti-S100B (30 μg of total protein extract), n = 3 animals per group. Densitometry is normalized to actin, and data are the mean percentage ± SE from three different animals per group. *p < 0.03, **p < 0.013.