IL-10RA expression ex vivo. (A) Representative Histograms showing IL-10RA MFI in CD4+Foxp3+ Treg and CD4+Foxp3− non-Treg cells in cord blood (CB), maternal blood (MB), and in nonpregnant (NP) women. A summary of MFI values within non-Treg and Treg cells are shown as individual dots from the three cohorts, CB (n = 20), MB (n = 19), and NP (n = 18), with the bar representing the mean. Paired t test, p values as indicated. (B) Correlation of IL-10RA and Bcl-2 expression in CD4+Foxp3+ Treg cells. Pearson’s correlation coefficient, r, and p values as indicated. (C) Summary plots with mean values, showing Bcl-2 MFI in Foxp3+ Treg cells in NP versus pregnant individuals (n = 20). (D) Correlation of Bcl-2 MFIs in CD4+Foxp3+ Treg cells between CB and corresponding MB (n = 19). Pearson’s correlation coefficient, r, and p values as indicated.