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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 15;190(12):6570–6578. doi: 10.4049/jimmunol.1102487

Figure 6. Overexpression of anti-IL-1β has no effect on IL-1β mRNA stability.

Figure 6

RAW 264.7 cells were stimulated with LPS for 4 hours, followed by 50μg/ml Actinomycin D treatment. Total RNA was extracted for real-time PCR. IL-1β (A) and TNFα (B) degradation, and anti-IL-1β (C) overexpression were determined by real-time qPCR.