Table 1.
Primer sequences used in the gene expression analysis. The genes monitored are used as markers for the pathways indicated. Jasmonate (JA), salicylic acid (SA), abscisic acid (ABA), and ethylene (ET)
| ID | Target Gen | Related pathway | Primer (5′–3′) |
|---|---|---|---|
| AF083253 | Multicystatin1 (MC) | JA inducible | GAGAATTTCAAGGAAGTTCAA |
| GGCTTTATTTCACACAGAGATA | |||
| K03291 | Proteinase inhibitor II1(PI II) | JA inducible | GAAAATCGTTAATTTATCCCAC |
| ACATACAAACTTTCCATCTTTA | |||
| M84801 | Prosystemin2(PS) | JA and ABA inducible | AATTTGTCTCCCGTTAGA |
| AGCCAAAAGAAAGGAAGCAAT | |||
| M69247 | Pathogenesis-related protein PR1a3 (PR1) | SA inducible | GTGGGATCGGATTGATATCCT |
| CCTAAGCCACGATACCATGAA | |||
| M83314 | Phenylalanine ammonia lyase2 (PAL) | SA biosynthesis | CGTTATGCTCTCCGAACATC |
| GAAGTTGCCACCATGTAAGG | |||
| X51904 | Desiccation protective protein3 (Le4) | ABA inducible | ACTCAAGGCATGGGTACTGG |
| CCTTCTTTCTCCTCCCACCT | |||
| NM001247876 | β-1,3-glucanase2 (gluB) | ET inducible | CCATCACAGGGTTCATTTAGG |
| CCATCCACTCTCTGACACAACT | |||
| X14449 | Elongation factor 1 α 4 (SlEF) | Housekeeping | GATTGGTGGTATTGGAACTGTC |
| AGCTTCGTGGTGCATCTC | |||
| XM_001560987.1 | β-tubulin5 | Quantification of B. cinereatubulin mRNA levels | CCGTCATGTCCGGTGTTACCAC |
| CGACCGTTACGGAAATCGGAA |