Figure 4.

Rho GTPases mediate the effect of PKA on YAP phosphorylation. (A) MDA-MB-231 cells were treated with 10 μM forskolin for 1 h, and cell lysates were subjected to immunoblotting. Phosphorylation of MLC2, CREB, and YAP was determined. (B) Flag-YAP was cotransfected into HEK293A cells with wild-type or constitutively active RhoA, and, after 16 h, cells were stimulated with 10 μM forskolin for 1 h before Western blotting. (C) Flag-YAP was cotransfected into HEK293A cells with or without GFP-tagged RhoGDI, and, after 16 h of incubation in serum-free medium, cells were treated with or without KT5720 for 1 h. Phosphorylation of Flag-YAP and endogenous TAZ was determined.