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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Protein Expr Purif. 2013 Apr 12;89(2):241–250. doi: 10.1016/j.pep.2013.04.001

Figure 2.

Figure 2

Investigating a natural degradation product of PglB for independent activity and enhanced expression. Top left: topology diagram for PglB based on available structural data (PDB 3RCE). The horizontal bar represents PglB with the protein sequence numbered along the bottom starting with the N-terminus. The location of each transmembrane helix is represented by a vertical bar and the thin black horizontal lines represent the location of the soluble regions (lumen on top, cytoplasm on bottom). Bottom left: Bars representing the various truncated constructs of PglB, with length aligned to X-axis of topology diagram. The name assigned to each construct is listed on the left along with the residues encompassed by that construct. Right: Western blots showing the degradation product of interest (indicated by the arrow) when stained with the anti-His antibody (C-terminal tag) but not when stained with the anti-T7 antibody (N-terminal tag).