. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Protein Expr Purif. 2013 Apr 12;89(2):241–250. doi: 10.1016/j.pep.2013.04.001

Table II.

Minor change in centrifugal spin significantly affects percent yield of purification step

	Fraction	Concentration (mg/mL)	Total protein (mg)	Rate (nmol/min)	Total activity (nmol/min)	Specific activity (nmol/min*mg)	Percent Yield	Fold Purification
Prep 1^a	lysate	9.5	380	1.2E-04	2.3E-01	6.12E-04	100	1.0
Prep 1^a	cleared lysate (10000 × g)	8.0	304	7.5E-05	1.4E-01	4.70E-04	61	0.8
Prep 2	lysate	12.9	514	351	4.7E+06	9.1E+03	100	1
Prep 2	cleared lysate (8000 × g)	6.7	270	318	4.2E+06	1.6E+04	91	2

^a.

Numbers are given for two PglB preparations representative of numbers seen in additional preps.