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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Protein Expr Purif. 2013 Apr 12;89(2):241–250. doi: 10.1016/j.pep.2013.04.001

Table III.

Comparison of several salt solutions in CEF salt wash efficiency.

Fraction Total Protein
(mg)		 Rate
(nmol/min)		 Total
activity		 Specific
activity		 Percent
Yield		 Fold
Purification
NaCl CEF (pre-wash) 143 5.6E-06 7.2E-02 5.0E-04 100 1.0
NaCl CEF (post-wash)a 75 2.5E-06 3.6E-02 4.7E-04 49 0.9
KCl CEF (pre-wash) 152 5.2E-06 6.8E-02 4.5E-04 100 1.0
KCl CEF (post-wash) 75 1.6E-06 2.5E-02 3.3E-04 36 0.7
NaCl + KCl CEF (pre-wash) 189 6.2E-06 8.0E-02 4.2E-04 100 1.0
NaCl + KCl CEF (post-wash) 75 3.6E-06 5.4E-02 7.2E-04 68 1.7
a.

The percent yield and fold-purification values for ‘post-wash’ fractions were determined relative to the pre-washed state only.