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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: Biochem Pharmacol. 2010 Jul 6;80(9):1309–1316. doi: 10.1016/j.bcp.2010.06.048

Figure 2. Inhibition of p38 enhances EF24-induced growth inhibition.

Figure 2

(A) Cells were treated with SB203580 or SB202190 for 48 hr. Cell viability was assessed using the SRB assay as the percentage of vehicle-treated (0.5% DMSO) control. (B, C) Combination treatment of A549 with EF24 (0.4µM) and SB203580 (B) or EF24 and SB202190 (C). The error bars in the experiments represent the standard deviation analyzed from three repeats. (D) A549 cells were pretreated with SB203580 for 1h before treatment with EF24 (10 µM) for 30 min. Cells were subjected to western blotting to access phosphorylation and protein expression of MAPKAPK-2 as a p38 substrate..