Fig.1.

Accuracy of the projection/reconstruction cycle. (A) Central slices through the ribosome phantom and reconstructions made from 5000 noiseless projections in RELION and in SPIDER using commands BP3F or BP3G. All four images are on the same grey-scale. (B) As in A, but showing reconstructions made from 5000 noisy projections. (C) FSC curves between the phantom and reconstructions obtained from the noiseless projections in RELION (black), SPIDER BP3G (grey) and SPIDER BP3F (dashed grey). (D) As in C, but for reconstructions from the noisy projections. Note the difference in the Y-axis range between C and D.