Fig.4.

Results obtained with the 3D auto-refine procedure for four cryo-EM data sets: (A) β-galactosidase, (B) GroEL, (C) hepatitis B capsid, and (D) recoated rotavirus. On the left are shown the resolution-dependent contributions to the orientability, i.e. to the sum inside the exponential in Eq. (8). On the right are shown representative pieces of reconstructed density (transparent yellow) with the corresponding fitted crystal structures inside (red). All maps were sharpened prior to visualization, using a B-factor of −1500, −750, −850 and −275 Å² for the β-galactosidase, GroEL, hepatitis B capsid, and recoated rotavirus reconstructions, respectively.