Table 2.

Refinement characteristics for four cryo-EM data sets.

	β-Galactosidase	groEL	Hepatitis B	Rotavirus
Sample characteristics
Size (MDa)	0.45	0.8	4	60
Symmetry	D2	D7	I	I
Microscopy settings
Microscope	FEI Polara G2	Jeol 3000SFF	Hitachi HF2000	FEI Tecnai F30
Voltage (kV)	80	300	200	300
Defocus range (μm)	1.2–2.7	1.9–3.2	1.0–2.0	1.2–2.9
Detector	Kodak SO163	Kodak SO163	Kodak SO163	Kodak SO163
Data characteristics
Image size (pixel2)	$100\times 100$	$128\times 128$	$220\times 220$	$400\times 400$a
Pixel size (Å)	2.93	2.12	2.00	2.40
Number of particles	50,330	5053	5403	3700
RELION parameters
Particle mask diameter (Å)	200	205	400	785
Initial low-pass filter (Å)	60	60	50	40
Initial angular sampling (°)	7.5	7.5	3.7	3.7
Local searches from (°)	1.8	1.8	0.5	0.5
Initial offset range (pixel)	6	6	6	6
Initial offset step (pixel)	1	1	1	1
RELION results
Wall-clock time (h)	13.6	2.0	8.2	41.5
Reported resolution (Å)	9.8	8.2	7.3	5.6
Resolution vs. X-ray (Å)	10.1	8.4	7.3	4.4b
Previous results
Refinement program	XMIPPc	EMAN2d	MRC	FREALIGNe
Reported resolution (Å)	13.9	8.4	7.4	$\approx$6
Resolution vs. X-ray (Å)	12.7	8.7	7.5	4.4b

^aThe original rotavirus particles were downscaled by a factor of 2 to reduce memory requirements.

^bAfter 13-fold non-icosahedral symmetry averaging, and for a masked region of the map comprising a single VP6 trimer.

^cThe results obtained using a gold-standard FSC version of the XMIPP projection matching protocol were used (Scheres et al., 2012).

^dResults from the EMAN2 tutorial (version 2011) were downloaded from http://blake.bcm.edu/emanwiki/Ws2011/Eman2.

^eFREALIGN results were downloaded from http://emlab.rose2.brandeis.edu/rota_recoated.