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Ionic strength dependence of NiSOD catalysis. Y9F- and WT-NiSOD were reacted with both NaCl (red-WT and yellow-Y9F) and NaClO4 (blue-WT and green-Y9F), while Y62F was only reacted with NaCl (black). Pulse radiolytic conditions were the same as those mentioned in Materials and Methods, except 0.5 M – 0.0156 M of either NaCl or NaClO4 was added to the solution being monitored to probe the effect of ionic strength.
