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. Author manuscript; available in PMC: 2013 Jun 24.

Published in final edited form as: Cancer Res. 2012 Feb 28;72(8):1975–1985. doi: 10.1158/0008-5472.CAN-11-2499

Effector responses to a tumor antigen were detected only after castration with concomitant Treg depletion in prostate tumor–bearing mice. A, Pten^−/− mice were analyzed 2.5 weeks after immunization (Immun) and castration (Cx) or sham treatment (Sx). Splenic tetramer-positive cells were enriched by magnetic bead selection followed by flow cytometric analysis. The number of tetramer-positive cells per spleen was calculated (bar graph; □, Sx + Immun; , Cx + Immun; ■, Cx + PC61 + Immun). *, P < 0.05 for Cx + PC61 + Immun versus Sx + Immun. B, IFN-γ–secreting splenocytes or PDLN cells in response to ex vivo UV-8101-RE stimulation were quantified using an ELISPOT assay for all 3 groups. C, the number of tetramer-positive cells per spleen 5 weeks after immunization and castration or sham surgery of Pten^−/− mice is shown. D, IFN-γ–secreting cells in spleen or PDLNs following ex vivo UV-8101-RE stimulation were quantified using ELISPOT assays for all 3 groups. *, P < 0.05; **, P < 0.001 for Cx + PC61 + Immun group versus Sx + Immun group or Cx + Immun group. Each group contained 3 to 5 animals, and the experiment was repeated once.

Inline graphic — Effector responses to a tumor antigen were detected only after castration with concomitant Treg depletion in prostate tumor–bearing mice. A, Pten^−/− mice were analyzed 2.5 weeks after immunization (Immun) and castration (Cx) or sham treatment (Sx). Splenic tetramer-positive cells were enriched by magnetic bead selection followed by flow cytometric analysis. The number of tetramer-positive cells per spleen was calculated (bar graph; □, Sx + Immun; , Cx + Immun; ■, Cx + PC61 + Immun). *, P < 0.05 for Cx + PC61 + Immun versus Sx + Immun. B, IFN-γ–secreting splenocytes or PDLN cells in response to ex vivo UV-8101-RE stimulation were quantified using an ELISPOT assay for all 3 groups. C, the number of tetramer-positive cells per spleen 5 weeks after immunization and castration or sham surgery of Pten^−/− mice is shown. D, IFN-γ–secreting cells in spleen or PDLNs following ex vivo UV-8101-RE stimulation were quantified using ELISPOT assays for all 3 groups. *, P < 0.05; **, P < 0.001 for Cx + PC61 + Immun group versus Sx + Immun group or Cx + Immun group. Each group contained 3 to 5 animals, and the experiment was repeated once.