A. Representative plate lysis assay with S. aureus strain Newman. Zones of clearing represent lysis of the lawn. 10 μg and 1 μg LysK is equivalent to 0.2 and 0.02 μmol, respectively; MW: 55.8 kD. 10 μg of Ply187AN is equivalent to 0.5 μmol; MW: 18.9 kD. 10, 1μg and 0.1 μg of Ply187AN-KSH3b is equivalent to 0.3, 0.03, and 0.003 μmol, respectively; MW: 30.6 kD. B. Turbidity reduction assays with S. aureus strain Newman. Results are presented as Specific activities (ΔOD600nm/μmol/min). The assay volume was 200 μl. Error bars represent SEM for three or more independent experiments. C. Representative MIC of LysK and purified Ply187 derivatives. The MIC assays were repeated four times with 3 replicates in each assay.“