Table 1.
Susceptibility of multiple bacterial strains to lysis by the parental enzyme LysK and the fusion protein Ply187AN-KSH3b
| Strain | Source | Susceptibilitya
|
|
|---|---|---|---|
| LysK | Ply187AN-KSH3b | ||
| S. aureus Newman | b | ++ | ++(+) |
| S. aureus MN8 | b | ++(+) | +++ |
| S. aureus SA113 | b | ++ | +++ |
| S. aureus Reynolds CP5 | b | ++(+) | +++ |
| S. aureus Newbould (305) | ATCC 29740 | ++(+) | +++ |
| S. aureus SA019 | c | ++(+) | +++ |
| S. aureus SA020 | c | ++(+) | ++(+) |
| S. aureus SA021 | c | ++ | ++ |
| S. aureus SA026 | c | ++ | ++(+) |
| S. aureus NRS382 (MRSA) | NRS 382 | +(+) | ++(+) |
| S. aureus NRS383 (MRSA) | NRS 383 | ++ | ++(+) |
| S. aureus NRS384 (MRSA) | NRS 384 | ++ | +++ |
| S. aureus NRS385 (MRSA) | NRS 385 | ++ | ++(+) |
| Staphylococcus chromogenes | d | ++(+) | ++(+) |
| Staphylococcus epidermidis | d | ++ | +++ |
| Staphylococcus hyicus | d | +++ | +++ |
| Staphylococcus simulans | d | ++(+) | +++ |
| Staphylococcus warneri | d | ++(+) | +++ |
| Staphylococcus xylosus | d | +++(+) | +++(+) |
| Streptococcus agalactiae | ATCC 27541 | − | − |
| Streptococcus dysgalactiae | e | − | + |
| Streptococcus uberis | f | − | − |
| Listeria monocytogenes Petite ScottA | ATCC 49594 | − | − |
| Rhodococcus equi | g | − | − |
| Lactobacillus amylovorus 4540 | h | − | − |
| Lactobacillus reuteri 14171 | h | − | − |
| E. coli H5 | i | − | − |
| E. coli DH5α | Invitrogen | − | − |
| Salmonella enteritidis | ATCC13076 | − | − |
| Klebsiella pneumoniae | j | − | − |
smallest amount of protein in a volume of 10 μl causing a lysis zone after overnight incubation: ++++, 0.1 pmol; +++, 1 pmol; ++, 10 pmol; +, 100 pmol. “(+)” represents a faint lysis zone; “− “, no lysis zone at the highest amount tested (100 pmol). Scores represent averaged results from two separate experiments.
Jean C. Lee, Channing Laboratory, Brigham and Women’s Hospital, Boston, MA, USA
Yasunori Tanji, Tokyo Institute of Technology, Yokohama, Japan; bovine mastitis isolates
Max Paape, ABBL, ANRI, ARS, USDA, Beltsville, MD, USA; bovine mastitis isolates
W.D. Schultze, BARC Dairy, Beltsville, MD, USA; isolated from a clinical case
Strain 0140; Dr. A.J. Bramley, Compton Laboratory, Newbury, United Kingdom; isolated from a clinical case
Strain 33701; Steve Giguere, College of Veterinary Medicine, University of Georgia, Athens, GA, USA
Ken Bischoff, ARS, Peoria, Ill, USA.
Manan Sharma, EMFSL, ANRI, ARS, USDA, Beltsville, MD, USA
Strain K-6; E.J. Carroll, Dept. Vet Med, University of California, Davis, CA, USA; cow 2612 clinical case