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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Biochemistry. 2013 Apr 16;52(17):2933–2948. doi: 10.1021/bi400180d

Figure 1.

Figure 1

The 2D-DIGE analysis of proteomic changes in whole lenses of 2-day-old mice induced by αA- and αB-crystallin gene deletions. (A) A 2D gel of lens proteins labeled with cyanine dyes derived from WT1 proteins labeled with Cy3, WT2 proteins labeled with Cy5, and DKO1 proteins labeled with Cy2. (B) Protein spots that were picked for analysis from the gel shown in (A). (C) A 2D gel of lens proteins labeled with cyanine dyes derived from WT3 proteins labeled with Cy2, WT4 proteins labeled with Cy3, and DKO2 proteins labeled with Cy5. (D) Protein spots that were picked for analysis from the gel shown in (C). Proteins were identified by tandem mass spectrometry and Mascot searches of spots that were differentially expressed. Quantitative image analysis and mass spectrometry data for identified proteins from this gel are listed in Table 1.