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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Biochemistry. 2013 Apr 16;52(17):2933–2948. doi: 10.1021/bi400180d

Figure 3.

Figure 3

(A) The 2D-DIGE analysis of proteomic changes in 70-day-old WT (WT5), 35-day-old WT (WT6), and 30-day-old DKO (DKO3) mouse lens epithelial fractions. A 2D gel of lens epithelial cell proteins labeled with cyanine dyes derived from WT5 proteins labeled with Cy2, WT6 proteins labeled with Cy3, and DKO3 proteins labeled with Cy5. Proteins were identified by tandem mass spectrometry and Mascot searches of spots that were differentially expressed. See Materials and Methods for additional details. (B) Protein spots that were picked for analysis are shown. Quantitative image analysis and mass spectrometry data for identified proteins from this gel are listed in Table 2. (C) Additional protein spots that were picked for analysis are shown. Identification of proteins in the analyzed spots is shown in Table 3.