Table 3.

Mascot search results for mouse lens proteins of WT (WT5, WT6) and DKO3 lens epithelial fractions to confirm protein deletion

Spot #	Protein ID	Accession # UNIPROT	# of assigned spectra	MW (kDa)	Fold change
					WT5 vs. WT6	WT5 vs. DKO3	WT6 vs. DKO3
2789	Glucose-6-phosphate	P06745	15	63	1.32	−4.81	−6.16
	isomerase,	P24549	3	54
	Retinal dehydrogenase 1,	Q03265	2	60
	ATP synthase subunit α mitochondrial,	P80315	2	58
	T-complex protein 1 subunit delta
3430	cryaa protein,	Q569M7	12	20	−3.07	−17.67	−5.59
	3’(2’),5’biphosphate nucleotidase 1	Q9Z0S1	8	33
4175	endoplasmic reticulum resident protein 29,	P57759	11	29	1.72	−2.57	−4.31
	cryaa protein	Q569M7	4	20
4474	cryaa protein,	Q569M7	19	20	−2.25	−27.82	−12.04
	β-A3/A1 crystallin,	Q9QXC6	7	25
	βB2-crystallin,	P62696	5	23
	βS-crystallin,	O35486	4	21
	βA2-crystallin	Q9JJV1	3	22
4485	βB2-crystallin,	P62696	42	23	−3.52	−7.04	−1.94
	cryaa protein	Q569M7	3	20
4644	alpha-crystallin B chain,	P23927	22	20	−2.17	−47.12	−21.08
	γS-crystallin,	O35486	21	21
	βB2-crystallin	P62696	4	23
4778	cryaa protein,	Q569M7	2	20	−3.4	−45.61	−13.04
	alpha-rystallin B	P23297	2	20
	chain,	Q64339		18
	calcium-regulated heat stable protein		2
4793	alpha-crystallin B chain,	P23927	16	20	−2.38	−9.4	−3.83
	γS-crystallin,	O35486	4	21
	γE-crystallin	Q03740	3	21
4871	cryaa protein	Q569M7	51	20	−2.22	−50.14	−21.93
5020	cryaa protein	Q569M7	185	20	−4.82	−35.85	−7.22
5532	alpha-crystallin B chain,	P23927	9	20	−1.82	−6.84	−3.65
	cryaa protein,	Q569M7	7	20
	histone H4,	P62805	4	11
	hemoglobin subunit α,	P01942	3	12
	peptidyl-propyl cis-trans isomerase FKBP1A,	P26883	3	15
	βB2-crystallin	P62696	2	23

WT5 proteins were labeled with Cy2, WT6 proteins were labeled with Cy3, and DKO3 proteins were labeled with Cy5, then analyzed on a single gel.