. Author manuscript; available in PMC: 2014 Apr 30.

Published in final edited form as: Biochemistry. 2013 Apr 16;52(17):2933–2948. doi: 10.1021/bi400180d

Table 4.

Molecular weight (M_w) and R_h of crystallins in WT and DKO mice

Age (days)	Genotype	M_w (kDa)	R_h (nm)	M_w (kDa)	R_h (nm)	M_w (kDa)	R_h (nm)	M_w (kDa)	R_h(nm)
		α-Crystallin		Additional peak or shoulder		β-Crystallin		γ-Crystallin
7	WT	682.082	8.140	n.d	n.d	43.690	3.283	19.295	2.278
117	WT	1141	9.818	n.d	n.d	54.647	3.195	23.988	2.290
9	DKO	n.d	n.d	327.946 (small peak near α)	^**	52.034	3.244	23.780	2.466
107	DKO	n.d	n.d	297.690 (shoulder before β)	5.141	70.677	3.371	26.737	2.731

Proteins from an adult DKO lenses were largely water-insoluble. Due to insufficient soluble protein, the R_h values from viscosity measurements for the DKO lenses were used. The DKO lens proteins exhibited a shoulder before the main β-crystallin peak (Figure 4C and D). An additional peak with an estimated molecular weight of > 10×10⁶ was observed in 9-day-old DKO lenses (Figure 4B).

R_h, hydrodynamic radius; n.d, not detected.

^**

R_h was approximated.