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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: Nature. 2012 Aug 30;488(7413):660–664. doi: 10.1038/nature11282

Figure 4. RSPO fusion products activate Wnt signalling.

Figure 4

a, Secreted RSPO fusion proteins detected by western blot in media from 293T cells transfected with expression constructs encoding the fusion proteins. The arrowhead indicates the expected product for RSPO 1–387. b, RSPO fusion proteins activate and potentiate Wnt signalling as measured using a luciferase reporter assay (see Supplementary Methods). Data shown are from conditioned media derived from cells transfected with the fusion constructs. Representative data from at least three experiments are shown. Error bars represent mean ± s.d. from three replicate experiments. EGFP, enhanced green fluorescent protein. c, Cartoon representing R-spondin-mediated Wnt signalling pathway activation. d, Quilt plot depicting RSPO fusions, somatic mutations, copy-number alteration and expression changes across a select set of Wnt-signalling pathway genes. Each column represents a tumour sample. Het, heterozygous; Hom, homozygous; WT, wild type.