Figure 8. Overexpression of TRPC6 T487A mutant in a smooth muscle cell line, A7r5, greatly inhibited endogenous AVP-induced TRPC6-like currents.

A, typical trace of whole-cell current induced by AVP (100 nm) in an empty vector-transfected A7r5 cell. Application of flufenamate (flu) caused clear potentiation of the current, which is the hallmark feature of a TRPC6 channel. B, overexpression of T487A into A7r5 cells significantly inhibited AVP-induced current, which still exhibited flu sensitivity. C, comparison of AVP-induced current density between empty vector and T487A mutant transfection.