Table II.

Clusters of Missense Perforin Mutations and Their Functional Assignment on the Protein Structure

Cluster no	Domain	Region	Function
Cluster 1	MACPF	Interface of perforin monomers	Oligomerization
Cluster 2	MACPF	Small clusters of α helices	Membrane-spanning
Cluster 3	C2	Ca binding domain	Membrane attachment

Residue numbers in clusters:Cluster 1: 38, 39, 45, 50, 54, 70, 73, 89, 91, 95, 102, 119, 123, 126, 149, 177, 183, 193, 198, 201, 215, 220, 221, 222, 224, 225, 232, 305, 306, 334, 345, 356, 357, 361Cluster 2: 157, 168, 170, 240, 252, 253, 261, 262, 279, 298, 299, 313, 317Cluster 3: 426, 429, 430, 435, 438, 450, 459, 481, 486, 491