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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Pharmacol Biochem Behav. 2013 Apr 18;108:28–43. doi: 10.1016/j.pbb.2013.04.005

Fig. 4. Saturation analysis of racemic [3H]mecamylamine binding to rat brain membranes in the absence (top) and presence of 1 mM S(−)nicotine (bottom).

Fig. 4

Saturation analysis was determined using a concentration range of 20–1500 nM of racemic [3H]-mecamylamine in both the absence (top) and presence of nicotine (1 mM; bottom). Nonspecific binding was measured with 100 μM (±)-mecamylamine. Data are expressed as fmol/mg protein and represent the mean ± S.E.M. of three independent experiments. Curves were generated using nonlinear regression for a one-site model. The inset illustrates the Scatchard transformation of the specific binding data.