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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Pharmacol Biochem Behav. 2013 Apr 18;108:28–43. doi: 10.1016/j.pbb.2013.04.005

Fig. 5. High concentrations of mecamylamine stereoisomers are required to compete for racemic [3H]-mecamylamine binding to rat brain membranes in the presence of 1 mM S(−)nicotine.

Fig. 5

Competitive binding assays were performed for racemic, S(+)- and R(−)-mecamylamine (1 nM - 1 mM) against 100 nM racemic [3H]-mecamylamine both in the absence (top) and presence of nicotine (1mM; bottom panel). Nonspecific binding was determined using 100 μM racemic mecamylamine. Data are expressed as fmol/mg protein and represent the mean ± SEM of three independent experiments. Curves were generated by nonlinear regression using a one-site model. Ki values are expressed as mean ± S.E.M. Ki values could not be calculated in the presence of nicotine.