Fig. 7.
In the long bones of DMP1Cre,CαR mice, genes associated with osteoblast differentiation are up-regulated while SOST expression is down-regulated. (A) Real-time PCR analysis of genes that are associated with osteoblast differentiation, (B) encode for negative regulators of Wnt signaling, and (C) targets of canonical Wnt pathway. RNA was extracted from the whole bone of femurs and tibiae. HKG denotes housekeeping genes. Runx2, osterix, and ALP expression are normalized to L19. Collagen 1α1 and osteocalcin are normalized to GAPDH. All data are mean ± SEM and collected from 3-month old female mice. n ≥ 4. T-test: # p < 0.05, ## p < 0.01.