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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: Gene. 2009 Nov 26;451(0):45–53. doi: 10.1016/j.gene.2009.11.010

Fig. 4.

Fig. 4

Deletion analysis of Rgs4 promoter in rabbit colonic smooth muscle cells (A) and human HEK293T cells (B) showing higher promoter activity in the proximal region and repressive activity in the distal region. Cultured cells were cotransfected with indicated Rgs4 promoter and various mutant vectors plus normalization vector. After 24 h, renilla and firefly luciferases were measured separately. Relative fold changes in renilla luciferase activity after normalization by firefly luciferase were expressed as compared with the promoter-less empty vector. Values represent the mean±SEM of 4 individual experiments.